改良自酸蚀粘接剂对龋影响牙本质粘接性能的影响

doi:10.3877/cma.j.issn.1674-1366.2015.04.007

中华口腔医学研究杂志(电子版) ›› 2015, Vol. 09 ›› Issue (04) : 295 -300. doi: 10.3877/cma.j.issn.1674-1366.2015.04.007

所属专题：文献；

基础研究

改良自酸蚀粘接剂对龋影响牙本质粘接性能的影响

李小龙¹, 覃峰¹, 钱宇¹, 尹映竹¹, 黄雪清²^,^†(

), 罗涛³(

)

^1. 510055　广州，中山大学光华口腔医学院·附属口腔医院，广东省口腔医学重点实验室
^3. 510140　广州医科大学附属口腔医院·广州口腔病研究所·口腔医学重点实验室

收稿日期:2015-03-25 出版日期:2015-08-01
通信作者: 黄雪清, 罗涛
基金资助:
教育部博士点基金(新教师类，20130171120121); 国家自然科学基金(青年科学基金项目，81300902); 广东省科技计划(2011B031300018); 中山大学青年教师培育计划(12ykpy62)

Effect of epigallocatechin-3-gallate to self-ectching adhisive to the bonding strengh of caries-affected dentin

Xiaolong Li¹, Feng Qin¹, Yu Qian¹, Yingzhu Yin¹, Tao Luo²(), Xueqing Huang³^,^†()

^1. Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China
^2. Stomatology Hospital of Guangzhou Medical University, Guangzhou Institute of Oral Disease, Key Laboratory of Oral Medicine, Guangzhou 510140, China

Received:2015-03-25 Published:2015-08-01
Corresponding author: Tao Luo, Xueqing Huang
About author:
Corresponding author: Huang Xueqing, Email: 84742261@qq.com, Tel: 020-83802805

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引用本文：

李小龙, 覃峰, 钱宇, 尹映竹, 黄雪清, 罗涛. 改良自酸蚀粘接剂对龋影响牙本质粘接性能的影响[J]. 中华口腔医学研究杂志(电子版), 2015, 09(04): 295-300.

Xiaolong Li, Feng Qin, Yu Qian, Yingzhu Yin, Tao Luo, Xueqing Huang. Effect of epigallocatechin-3-gallate to self-ectching adhisive to the bonding strengh of caries-affected dentin[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(04): 295-300.

目的

探讨表没食子儿茶素没食子酸酯(EGCG)改良后自酸蚀粘接剂可乐丽Clearfil SE Bond对龋影响牙本质即刻粘接性能影响。

方法

选取中龋离体牙20颗，在龋指示剂下去除龋感染牙本质，保留龋影响牙本质。将EGCG配制成100、200、300 μg/ml Clearfil SE Bond粘接剂溶液及水溶液，以不添加EGCG粘接剂及水溶液作为对照组。微拉伸试件分组按照Clearfil SE Bond说明书指导进行粘接，上方堆塑5~6 mm Z350树脂。使用慢速线性切割机沿牙齿长轴进行切割，形成约1 mm × 1 mm试件，进行微拉伸实验。显微硬度试件分组浸泡于EGCG水溶液中24 h后测试显微硬度。使用SPSS 22.0统计学软件，在One-Way ANOVA模式下进行统计学分析。

结果

EGCG处理后龋影响牙本质的粘接强度及显微硬度均明显提高，差异有统计学意义(F_粘接强度=24.554，P_粘接强度 < 0.05；F_显微硬度=20.418，P_显微硬度 < 0.05)。

结论

EGCG可增强龋影响牙本质的即刻粘接强度及显微硬度。

关键词: 表没食子儿茶素没食子酸酯, 龋影响牙本质, 粘接强度, 显微硬度

Objective

To explore the effect of the modified Epigallocatechin-3-Gallate(EGCG)on self-etch Clearfil SE Bond bonding strength to caries-affected dentin.

Methods

Twenty extracted human teeth with medial coronal carious lesions extending into middle dentin were used. The softened carious dentin was removed by Caries Marker. EGCG was incorporated at a ratio of 100, 200, and 300 μg/ml into water solution and a dental adhesive, Clear SE Bond. Caries-affected dentin was treated with EGCG water solution for 24 hours before micro-hardness test. And Microtensile bond strength(μTBS)test was used to evaluate the bonding strength to caries-affected dentin treated by Clearfil SE Bond with different EGCG concentration. Statistics analysis software SPSS 22.0 was used to evaluate the difference by One-Way ANOVA.

Result

Micro-hardness and bonding strength of caries-affected dentin are both significantly increased when treated with EGCG(F_{bonding strength}=24.554, P_{bonding strength} < 0.05; F_{micro-hardness}=20.418, P_{micro-hardness} < 0.05).

Conclusion

EGCG can significantly increase the micro-hardness and bonding strength of caries-affected dentin.

Key words: Epigallocatechin-3-gallate, Caries-affected dentin, Bonding strength, Micro-hardness

表1 添加不同浓度EGCG后Clearsil SE Bond对龋影响牙本质微拉伸强度(MPa，±s)

组别	微拉伸强度
对照组	24.28 ± 2.86
EGCG 100 μg/ml组	30.15 ± 2.92^a
EGCG 200 μg/ml组	36.95 ± 4.02^ab
EGCG 300 μg/ml组	37.44 ± 6.60^ab

表1 添加不同浓度EGCG后Clearsil SE Bond对龋影响牙本质微拉伸强度(MPa，±s)

组别	微拉伸强度
对照组	24.28 ± 2.86
EGCG 100 μg/ml组	30.15 ± 2.92^a
EGCG 200 μg/ml组	36.95 ± 4.02^ab
EGCG 300 μg/ml组	37.44 ± 6.60^ab

表2 不同浓度EGCG处理后龋影响牙本质表明显微硬度(MPa，±s)

组别	显微硬度
对照组	54.89 ± 5.10
EGCG 100 μg/ml组	61.80 ± 3.30^a
EGCG 200 μg/ml组	69.47 ± 6.06^ab
EGCG 300 μg/ml组	70.13 ± 3.29^ab

表2 不同浓度EGCG处理后龋影响牙本质表明显微硬度(MPa，±s)

组别	显微硬度
对照组	54.89 ± 5.10
EGCG 100 μg/ml组	61.80 ± 3.30^a
EGCG 200 μg/ml组	69.47 ± 6.06^ab
EGCG 300 μg/ml组	70.13 ± 3.29^ab

图1 EGCG水溶液处理后龋影响牙本质表面显微压痕(体视显微镜× 10)

图2 EGCG处理后龋影响牙本质粘接断裂界面的扫描电镜图

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