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中华口腔医学研究杂志(电子版) ›› 2015, Vol. 09 ›› Issue (04) : 272 -276. doi: 10.3877/cma.j.issn.1674-1366.2015.04.003

所属专题: 文献

基础研究

粘着斑激酶和血管内皮生长因子在唾液腺腺样囊性癌中的表达及意义
龚攀1, 邝燕好2, 欧阳可雄3, 陈凯4, 李劲松5,()   
  1. 1. 510095 广州医科大学附属肿瘤医院口腔科
    3. 510140 广州医科大学附属口腔医院口腔颌面外科
    4. 510080 广州,广东药学院附属第一医院口腔科
    5. 510120 广州,中山大学孙逸仙纪念医院口腔颌面外科
  • 收稿日期:2015-05-07 出版日期:2015-08-01
  • 通信作者: 李劲松
  • 基金资助:
    国家自然科学基金(81272951、81472521); 广州医科大学青年科研项目(2014A37)

Expression of focal adhesion kinase and vascular endothelial growth factor in salivary adenoid cystic carcinoma and their significances

Pan Gong1, Kexiong Ou-Yang2, Kai Chen3, Yanhao Kuang4, Jinsong Li5,()   

  1. 1. Department of Stomatology, the Cancer Center of Guangzhou Medical University, Guangzhou 510095, China
    2. Department of Oral and Maxillofacial Surgery, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China
    3. Department of Stomatology, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China
    5. Department of Oral and Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
  • Received:2015-05-07 Published:2015-08-01
  • Corresponding author: Jinsong Li
  • About author:
    Corresponding author: Li Jinsong, Email:
引用本文:

龚攀, 邝燕好, 欧阳可雄, 陈凯, 李劲松. 粘着斑激酶和血管内皮生长因子在唾液腺腺样囊性癌中的表达及意义[J/OL]. 中华口腔医学研究杂志(电子版), 2015, 09(04): 272-276.

Pan Gong, Kexiong Ou-Yang, Kai Chen, Yanhao Kuang, Jinsong Li. Expression of focal adhesion kinase and vascular endothelial growth factor in salivary adenoid cystic carcinoma and their significances[J/OL]. Chinese Journal of Stomatological Research(Electronic Edition), 2015, 09(04): 272-276.

目的

探讨粘着斑激酶(FAK)和血管内皮生长因子(VEGF)在唾液腺腺样囊性癌(SACC)中的表达及意义。

方法

采用SP免疫组织化学方法检测40例SACC、15例正常唾液腺组织中FAK、VEGF、CD34的表达情况。实验数据采用SPSS 19.0统计软件进行Mann-Whitney U检验、方差分析和Fisher′s精确经验,FAK和VEGF表达进行相关分析。

结果

40例SACC组织中FAK阳性表达率为87.5%(35/40),对照组为33.33%(5/15),Z=-4.44,P= 0.001;VEGF阳性表达率为90%(36/40),对照组为26.67%(4/15),Z=-4.84,P=0.001;CD34阳性表达率为85%(34/40),对照组为40%(6/15),Z=-4.18,P=0.002,差异均有统计学意义;FAK和VEGF在SACC中的表达与肿瘤血管生成之间具有关联(FFAK=8.86、PFAK=0.01;FVEGF=8.18、PVEGF=0.01);FAK和VEGF在SACC中的表达与肿瘤的临床分期以及远处转移有关联(P < 0.05),与患者的性别、年龄、肿瘤发生部位和大小、神经侵犯、病理分级、复发情况和淋巴结转移差异无统计学意义(P > 0.05);FAK和VEGF在SACC中的表达存在正相关性(r=0.479,P=0.002)。

结论

FAK和VEGF与SACC的发展及侵袭、转移有关联。

Objective

To investigate the expression of focal adhesion kinase(FAK)and vascular endothelial growth factor(VEGF)in salivary adenoid cystic carcinoma(SACC)and their significances.

Methods

The expressions of FAK, VEGF and CD34 in 40 cases of SACC and 15 cases of normal salivary gland tissue were detected with immunohistochemical method. SPSS 19.0 software was used for Mann-Whitney U, Chi-square test, Fisher′s exact test and correlation analysis.

Results

The expression of FAK was seen in 87.5% of SACC and 33.33% in the control group, Z=-4.44, P=0.001; The expression of VEGF was 90% and 26.67% respectively, Z=-4.84, P=0.001; The expression of CD34 was 85% and 40% respectively, Z=-4.18, P=0.002. The differences were significant(P < 0.05). The expression of FAK and VEGF in SACC was associated with tumor angiogenesis(FFAK=8.86、PFAK=0.01; FVEGF=8.18、PVEGF=0.01). The expression of FAK and VEGF was significantly associated with clinical stage and metastasis in the samples(P < 0.05), but did not correlate with sex, age, tumor diameter and pathological grade(P > 0.05). The expression of FAK was positively correlated with the expression of VEGF. The coefficient of correlation was 0.479, P=0.002.

Conclusion

The expression of FAK and VEGF is significantly associated with tumor clinical stage clinical stage and metastasis.

图9 正常唾液腺组织CD34低表达(SP × 400)
表1 FAK、VEGF在SACC中不同表达强度下MVD的计数对比
表2 FAK、VEGF和唾液腺腺样囊性癌临床病理特征的关系[例(%)]
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