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中华口腔医学研究杂志(电子版) ›› 2007, Vol. 01 ›› Issue (02) : 78 -82. doi: 10.3877/cma.j.issn.1674-1366.2007.02.005

基础研究

survivin在尼古丁抑制顺铂诱导的舌鳞癌细胞凋亡中的作用
许建辉1, 黄洪章1,, 潘朝斌1, 张彬1, 王建广1, 张磊涛1   
  1. 1.510120 广州,中山大学附属第二医院口腔颌面外科·中山大学口腔医学研究所
  • 收稿日期:2007-02-18 出版日期:2021-02-01
  • 通信作者: 黄洪章

The role of survivin in nicotine-mediated inhibition of apoptosis induced by cisplatin in tongue squamous cell carcinoma cells

Jian-hui XU1, Hong-zhang HUANG1,, Chao-bin PAN1, Bin ZHANG1, Jian-guang WANG1, Lei-tao ZHANG1   

  1. 1.Department of Oral & Maxillofacial Surgery, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China
  • Received:2007-02-18 Published:2021-02-01
  • Corresponding author: Hong-zhang HUANG
引用本文:

许建辉, 黄洪章, 潘朝斌, 张彬, 王建广, 张磊涛. survivin在尼古丁抑制顺铂诱导的舌鳞癌细胞凋亡中的作用[J/OL]. 中华口腔医学研究杂志(电子版), 2007, 01(02): 78-82.

Jian-hui XU, Hong-zhang HUANG, Chao-bin PAN, Bin ZHANG, Jian-guang WANG, Lei-tao ZHANG. The role of survivin in nicotine-mediated inhibition of apoptosis induced by cisplatin in tongue squamous cell carcinoma cells[J/OL]. Chinese Journal of Stomatological Research(Electronic Edition), 2007, 01(02): 78-82.

目的

探讨凋亡抑制蛋白survivin 在尼古丁抑制顺铂诱导的舌鳞癌细胞凋亡中的作用。

方法

口腔鳞癌细胞株Tca8113 无血清培养24 h,使其处于相对生长停滞状态,建立顺铂诱导凋亡模型, 以终浓度为1 μmol/L 的尼古丁进行干预,通过TUNEL 检测、免疫印迹分析和RNA 干扰技术等方法,观察细胞凋亡。

结果

尼古丁抑制顺铂诱导的Tca8113 细胞的凋亡。 凋亡抑制蛋白survivin 参与尼古丁抑制肿瘤细胞的凋亡过程,小干扰RNA 导致的survivin 沉默阻碍了尼古丁对顺铂诱导的细胞凋亡的抗凋亡作用。同时,尼古丁抗凋亡作用需要Akt 信号通路,Akt 拮抗剂LY294002 削弱尼古丁的抗凋亡作用并抑制尼古丁诱导的survivin 表达。

结论

尼古丁可能负性影响化疗药物诱导的细胞凋亡,survivin 在尼古丁抗细胞凋亡中起重要作用,Akt 通路对尼古丁的功能发挥是必需的。

Objective

Oral cancer demonstrates a strong epidemiological association with smoking. Nicotine, a major component of cigarette smoke, can regulate cell proliferation and angiogenesis and suppress apoptosis induced by chemotherapeutic drugs. But little is known about the effect of nicotine on oral cancer cell apoptosis.

Methods

Cisplatin-induced apoptosis of human oral cancer cells (Tca8113) were rendered quiescent by serum starvation for 24 h. The effect of nicotine on apoptosis was observed by TUNEL assay, Western blotting and RNA interfering technology.

Results

Nicotine inhibited apoptosis induced by cisplatin in Tca8113 cells. Survivin took part in the inhibiting apoptosis process of nicotine in human oral cancer Tca8113 cells. Depletion of survivin by the siRNA ablated the protective effects of nicotine against cisplatin-induced apoptosis. Meanwhile, Akt pathway is necessary for the antiapoptotic effect of nicotine. LY294002 (an antagonist of Akt pathway) ablated the antiapoptotic effect of nicotine and inhibited the induction of survivin by nicotine in Tca8113 cells.

Conclusions

Exposure to nicotine might negatively impact the apoptotic potential of chemotherapeutic drugs. Survivin plays a key role in the antiapoptotic activity of nicotine. The Akt pathway may be required for nicotine function.

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