目的

研究在小鼠颌骨发育过程中长链非编码RNA（lncRNA）的表达谱变化情况。

方法

利用lncRNA-seq 测序技术检测孕18 d 及出生后14 d 的C57 小鼠下颌骨组织样本中的lncRNA 表达谱差异，经对原始数据进行预处理达到均一化后，筛选出差异表达lncRNA，进行分析。

结果

孕18 d 与出生后14 d 的C57 小鼠下颌骨组织样本相比较，2 倍以上变化并有显著差异（FDR≤0.001）的lncRNA，确定为差异表达lncRNA。 2 倍以上变化的共6617 条，占所有lncRNA 的17.16%；2 倍以上升高的共3720 条；2 倍以上降低的共2897 条；5 倍以上升高的共714 条；5 倍以上降低的共288 条；10 倍以上升高为共645 条；10 倍以上降低的共211 条。

结论

孕18 d 与出生后14 d 的C57 小鼠下颌骨组织样本相比较，lncRNA 表达谱发生显著变化。 提示差异性表达的lncRNA可能参与了小鼠颌骨发育的调控。

Objective

To analyze the expression of long non-coding RNA （lncRNA） in the jaw development process of mice.

Methods

lncRNA-seq sequencing technology was used to inspect the difference of lncRNA expression profile between C57 mice of embryonic day 18 and 2 weeks after birth.The lncRNAs with different expression levels were screened out after pretreatment and homogenization of raw data. The hierarchical clustering and volcano scatter diagram analysis were performed.

Results

Mandibular tissues of the C57 mice's of embryonic day 18 and 2 weeks after birth were compared. 6617 lncRNAs showed more than 2 times difference （FDR≤0.001） between the two groups were recognized as lncRNAs with differential expression， accounting for 17.16% of all lncRNAs. Among thses lncRNAs，3720 increased more than 2 times and 2897 reduced more than 2 times， 714 increased more than 5 times and 288 reduced more than 5 times， 645 increased more than 10 times and 211 reduced more than 10 times.

Conclusion

In the jaw development process of mice， the lncRNA expression changes significantly， suggesting that lncRNAs may participate in the regulation of the jaw development.