目的

探讨不同浓度的富血小板血浆（PRP）支架在体内诱导牙髓组织再生的能力。

方法

将小型猪乳牙牙根段进行化学预备，采用二次离心法制备PRP，根据注入根管中的成分不同将研究分为4 组：（1）阴性对照组，即全血组；（2）100% PRP 组；（3）50% PRP 组；（4）空白组，即空的牙根段；每组5 个样本，分别植入裸鼠背部皮下，于术后5 周处死动物，取出样本进行组织学观察。

结果

植入5 周后，100% PRP 组根管内充满了炎性细胞，50% PRP 组根管内有少量牙髓样的组织生成。

结论

合适浓度的PRP 作为生物支架在体内再生牙髓样的组织是可行的。

Objective

To evaluate the in vivo dental pulp regeneration capacity of various concentration of the platelet-rich plasma （PRP）.

Methods

The tooth roots of minipigs were prepared by chemical methods. PRP was extracted by two-step centrifugation. The roots were divided into four groups according to the kinds of scaffold injected into the root canals （5 roots each group）： （1）control group， whole blood； （2）100% PRP； （3）50% PRP； （4）blank group： root fragments with empty canal space. The roots were implanted subcutaneously in nude mice. The animals were sacrificed after 5 weeks.The samples were taken for histological examination.

Results

The newly formed pulp-like tissues were observed in the roots which filled 50% PRP scaffolds in canals. The group of 100% PRP scaffolds were full of inflammatory cells.

Conclusions

It is feasible for the platelet-rich plasma with proper concentration as scaffolds to induce dental pulp regeneration in vivo.