目的

检测正常口腔黏膜、口腔黏膜白斑和口腔鳞状细胞癌（OSCC）中p53 和增殖细胞核抗原（PCNA）表达，探讨p53 和PCNA 在OSCC 癌变过程中的规律及相关性。

方法

口腔黏膜白斑标本20 例、OSCC 标本31 例及正常口腔黏膜10 例，采用免疫组化SP 法检测其p53和PCNA 的表达，应用SPSS 11.0 软件包对结果进行χ² 检验、单因素方差分析和线性回归分析。

结果

p53 在正常黏膜组、白斑组与鳞状细胞癌组的表达率分别为0%、35%和77.4%，PCNA 在上述三组中表达率分别为30%、70%和96.8%，两种表达的组间比较差异有统计学意义（P＜0.05）；p53 蛋白与PCNA 表达呈正相关（P＜0.01）。

结论

p53 基因和PCNA 表达水平均与OSCC 发展进程密切相关。

Objective

To investigate the pattern and correlation of p53 and proliferating cell nuclear antigen （PCNA） in canceration of oral squamous cell carcinoma （OSCC）， p53 and PCNA in the normal oral mucosa， oral leukoplakia and OSCC were detected.

Methods

Twenty leukoplakia of oral mucosa， 31 OSCC and 10 oral normal mucosa were tested. The expressions of p53 and PCNA were detected by immunohistochemical technique （SP method）. The data was analyzed by Fisher′s exact test， one-way ANOVA and linear regression analysis using SPSS 11.0 soltware package.

Results

The rates of p53 expression in normal oral mucosa， leukoplakia and OSCC were 0%， 35% and 77.4% respectively. The rates of PCNA in the above-mentioned three groups were 30%， 70% and 96.8% respectively. There were significant differences of the expressions of p53 and PCNA among the oral normal mucosa， oral leukoplakia and OSCC （P＜0.05）； The expressions of p53 was linear positively correlated to which of PCNA （P＜0.01）.

Conclusion

The over expression of p53 and PCNA were closely correlated to the process of malignant changes of oral mucosal precancerous lesions.