目的

建立稳定的人舌鳞状细胞癌（TSCC）严重联合免疫缺陷（SCID）小鼠异种移植瘤模型， 初步研究其组织形态学以及生物学行为。

方法

将未经治疗的新鲜人TSCC标本移植于SCID 小鼠腹部皮下，当肿瘤生长至10 ～15 mm 时，取出移植瘤再次移植传代，对比研究各代移植瘤的组织学形态，原位杂交以及免疫组织化学法检测肿瘤组织的来源。

结果

75%（8/12）标本成功建立起稳定移植瘤模型，最长传至第5 代。 组织形态学对比显示移植瘤保持了原代瘤的组织学特征和异质性；免疫组织化学证实肿瘤细胞的人源性和基质细胞的宿主源性。

结论

成功建立人TSCC SCID 小鼠异种移植瘤模型，较好的保持了原代肿瘤的特性。

Objective

The purpose of this study was to establish and characterize the model of human tongue squamous cell carcinoma （TSCC） in severe combined immunodeficiency（SCID） mouse.

Methods

Fresh untreated samples of TSCC were implanted subcutaneously in SCID mice.When xenografts reach 10-15 mm in diameter， they were harvested and cutted into small piece and re-implanted into mice.Samples of the original tumor as well as tumor from each passage were prepared for histology evaluation， in situ hybridization and immunohistochemical analysis.

Results

75%（8/12） of the xenografts grew enough to allow passage to subsequent SCID mice， the longest was implant to 5th passage.Histological analysis of H&E section reveal that the xenograft maintain the histology and heterogeneity of the original tumor，Immunohistochemical confirm the human original of the tumor and the host original of the stromal elements.

Conclusion

This model recapitulates many important features of original tumor， it should provide a valuable platform for cancer research.