中华口腔医学研究杂志(电子版) ›› 2009, Vol. 3 ›› Issue (06) : 588 -592. doi: 10.3877/cma.j.issn.1674-1366.2009-06-003 × 扫一扫
基础研究
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Dai-you WANG1,†(), Nuo ZHOU1, Bin ZHANG1, Xin-ping LU1, Shou-rong FANG1, Lei JIANG1
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王代友, 周诺, 张斌, 陆新萍, 方首镕, 蒋磊. 突变型Nbs1 基因转染对头颈癌放疗敏感性影响的实验研究[J/OL]. 中华口腔医学研究杂志(电子版), 2009, 3(06): 588-592.
Dai-you WANG, Nuo ZHOU, Bin ZHANG, Xin-ping LU, Shou-rong FANG, Lei JIANG. The experimental study on enhancive radio-sensitivity of head ＆ neck cancer cells after transfected with mutant Nbs1[J/OL]. Chinese Journal of Stomatological Research(Electronic Edition), 2009, 3(06): 588-592.
目的
探讨突变型Nbs1 基因转染对头颈癌细胞放射敏感性的影响。
方法
用突变型Nbs1 腺病毒载体转染头颈癌细胞株JHU006，同时给予2 Gy Co60 γ 射线照射，24 h 后用MTT 法测定细胞生长曲线，以及中性彗星法检测癌细胞DNA 双链断裂程度。
结果
JHU006细胞株转染突变型Nbs1 基因并配合放射治疗后，癌细胞处于生长停滞状态；中性彗星实验显示，该组癌细胞平均尾力矩值明显增大。
结论
突变型Nbs1 基因转染可以抑制头颈部DNA双链断裂癌细胞的损伤修复功能，因而显著增加了癌细胞放疗敏感性。
Objective
To determine the radio-sensitivity of head ＆ neck cancer cells after transfected with mutant Nbs1 gene.
Methods
JHU006 cells were transfected with mutant Nbs1 and then irradiated with 2 Gy of Co60 γ-ray for 24 hours. The MTT method was applied to determine the growth curve of head ＆ neck cancer cells JHU006 and Neutral comet assay was used for observing the cells' DNA Double strain breaks.
Results
After the treatment， JHU006 cells growth was almost at a standstill and the values of the mean rail moment remarkably increased.
Conclusions
Transfected with mutant Nbs1 gene， Head ＆ neck cancer cells' radiosensitivity significantly increased attribute to the inhibition of repair function for cancer cells DNA double strain breaks.