中华口腔医学研究杂志(电子版) ›› 2009, Vol. 3 ›› Issue (06) : 581 -587. doi: 10.3877/cma.j.issn.1674-1366.2009-06-002 × 扫一扫
基础研究
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Zhi-xiang PENG1,†(), Ruiz Teresa1, Fives-Taylor Paula1, Wu Hui1
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彭志翔, Teresa Ruiz, Paula Fives-Taylor, Hui Wu. 口腔副溶血链球菌Fap1 非依赖性菌毛的电镜观察及相关体外黏附实验[J/OL]. 中华口腔医学研究杂志(电子版), 2009, 3(06): 581-587.
Zhi-xiang PENG, Ruiz Teresa, Fives-Taylor Paula, Wu Hui. TEM observation of a non-Fap1 dependent fimbriae in Streptococcus parasanguinis and relevant in vitro binding assay[J/OL]. Chinese Journal of Stomatological Research(Electronic Edition), 2009, 3(06): 581-587.
目的
探讨副溶血链球菌表面Fap1 非依赖性短菌毛结构的形态学表现及其在细菌黏附中的作用。
方法
通过插入置换法构建Fap1 蛋白系列缺陷株以去除Fap1 依赖性长菌毛结构,电镜下观察细菌表面结构的形态学变化;系列缺陷株的黏附功能通过唾液包被的羟基磷灰石体外黏附实验加以评价。
结果
所有Fap1 蛋白形成缺陷株的菌体表面存在一种短菌毛结构,该种Fap1 非依赖性菌毛垂直于细胞表面呈稀疏分布,长度不大于100 nm;体外黏附实验表明仅存在短菌毛结构的系列缺陷株的黏附功能与野生株相比有明显下降,但仍保持19% ~27%的黏附能力。
结论
副溶血链球菌表面Fap1 非依赖性短菌毛在细菌的黏附活动中仅起一定辅助作用。
Objective
To investigate the S.parasanguinis cell surface morphological characteristics of non-Fap1 dependent short fimbriae and its possible role in bacterial adhesion.
Methods
An insertional replacement was carried out to construct a series of Fap1 defect mutants, thus the Fap1 dependent long fimbriae structure was removed. The bacterial surface morphological change was observed with transmission electron microscope (TEM); The adhesion capacities of the mutants were tested by using an in vitro saliva-coated hydroxyapatite (SHA)model.
Results
The non-Fap1 dependent short fimbriae exist on the cell surface of all Fap1 defect mutants. This kind of short fimbriae were perpendicular to cell surface and distributed sparsely with length less than 100 nm. In vitro SHA binding assay showed the mutants with short fimbriae had an obvious decrease in adhesion ability while comparing with that of the wild type strain. But they still remained 19% ~ 27% binding functions.
Conclusion
The non-Fap1 dependent short fimbriae merely play some minor role in S.parasanguinis bacterial adhesion activity.