切换至 "中华医学电子期刊资源库"

中华口腔医学研究杂志(电子版) ›› 2007, Vol. 01 ›› Issue (01) : 21 -24. doi: 10.3877/cma.j.issn.1674-1366.2007.01.007

基础研究

白介素-1β 与白介素-10 在慢性牙周病中的表达研究
任蕾1, 金力坚1,, 梁惠强1   
  1. 1.香港大学菲腊牙学院牙周科
  • 收稿日期:2007-01-11 出版日期:2007-02-01
  • 通信作者: 金力坚

The in vivo expression patterns of IL-1β and IL-10 in chronic periodontitis

Lei REN1, Lijian JIN,1, Wai-keung LEUNG1   

  1. 1.Department of Periodontology, Prince Philip Dental Hospital, 34# Hospital Road,Hong Kong, China
  • Received:2007-01-11 Published:2007-02-01
  • Corresponding author: Lijian JIN
引用本文:

任蕾, 金力坚, 梁惠强. 白介素-1β 与白介素-10 在慢性牙周病中的表达研究[J/OL]. 中华口腔医学研究杂志(电子版), 2007, 01(01): 21-24.

Lei REN, Lijian JIN, Wai-keung LEUNG. The in vivo expression patterns of IL-1β and IL-10 in chronic periodontitis[J/OL]. Chinese Journal of Stomatological Research(Electronic Edition), 2007, 01(01): 21-24.

目的

描述白介素-1β(IL-1β)与白介素-10(IL-10)在人牙龈组织中的表达方式,并研究这两种细胞因子在正常及病理牙周组织中的相互关系。

方法

从24 例慢性牙周病患者中取得牙周袋组织(PoTs), 炎性结缔组织(ICTs) 及临床健康型牙龈组织(HT-Ps),7 例牙周健康组织(HT-Cs) 作为对照组。

结果

IL-1β 主要表达在牙龈上皮基底细胞的核膜表面及上层细胞的细胞浆中;而IL-10 则主要表达在结缔组织的细胞间隙中。 IL-1β 在PoT 和ICT 中的水平比HT-P中显著增多,而IL-10 则表现为相反的表达水平。 二者呈现出显著的负相关关联。

结论

炎性细胞因子和抗炎细胞因子的相互作用对于研究牙周病的病因是很关键的。

Objective

To describe and characterize the in vivo expression profile of IL-1β and IL-10 in various categories of human gingival tissues and investigate the correlations between these two cytokines in periodontal health and disease.

Methods

Gingival biopsies were collected from 24 subjects with chronic periodontitis, including periodontal pocket tissues (PoTs), inflamed connective tissues (ICTs) and clinically healthy gingival tissues (HT-Ps), and from 7 periodontally healthy subjects as controls (HT-Cs). The expression of IL-1β and IL-10 was detected by immunohistochemistry.

Results

IL-1β mainly expressed on the surface of nuclear membrane of baser layer cells as well as in the cytoplasm of upper cells of epithelium. While IL-10 mainly expressed in the intercellular spaces of connective tissues beneath the gingival epithelium. Within the patients, the expression levels of IL-1β were significantly higher in ICTs compared to those of HT-Ps (Area% and Cell/Area). PoTs also exhibited significantly higher expression of IL-1β than HT-Ps (Cell/Area),while the mean expression levels of IL-10 showed the contrast results. Furthermore, significantly negative correlations were detected between IL-1β levels and IL-10 levels in overall tissues.

Conclusions

These results suggested that the dynamic interactions between pro- and anti-inflammatory cytokines might be crucial in periodontal pathogenesis.

表1 在各种牙龈组织中IL-1β 的表达水平
表2 在各种牙龈组织中IL-10 的表达水平
表3 在各种牙龈组织中IL-1β 和IL-10 的相关分析
1
Offenbacher S. Periodontal diseases: pathogenesis. Ann Periodontol, 1996,1(1):821-878.
2
Page RC, Offenbacher S, Schroeder HE, et al. Advances in the pathogenesis of periodontitis: summary of developments,clinical implications and future directions. Periodontol, 2000,1997,14:216-248.
3
Smith MA, Braswell LD, Collins JG, et al. Changes in inflammatory mediators in experimental periodontitis in the rhesus monkey. Infect Immun, 1993,61(4):1453-1459.
4
Hillmann G, Hillmann B, Geurtsen W. Immunohistological determination of interleukin-1 beta in inflamed human gingival epithelium. Arch Oral Biol, 1995,40(4):353-359.
5
Feldner BD, Reinhardt RA, Garbin CP, et al. Histological evaluation of interleukin-1 beta and collagen in gingival tissue from untreated adult periodontitis. J Periodont Res, 1994,29(1):54-61.
6
Kornman KS, Crane A, Wang HY, et al. The interleukin-1 genotype as a severity factor in adult periodontal disease. J Clin Periodontol, 1997,24(1):72-77.
7
Gemmell E, Marshall RI, Seymour GJ. Cytokines and prostaglandins in immune homeostasis and tissue destruction in periodontal disease. Periodontol, 2000,1997,14:112-143.
8
Gemmell E, Kjeldsen M, Yamazaki K, et al. Cytokine profiles of Porphyromonas gingivalis-reactive T lymphocyte line and clones derived from P. gingivalis-infected subjects. Oral Dis,1995,1(3):139-146.
9
Huang WT, Lu HK, Chou HH, et al. Immunohistochemical analysis of Th1/Th2 cytokine profiles and androgen receptor expression in the pathogenesis of nifedipine-induced gingival overgrowth. J Periodont Res, 2003,38(4):422-427.
10
Jiang Y, Magli L, Russo M. Bacterium-dependent induction of cytokines in mononuclear cells and their pathologic consequences in vivo. Infect Immun, 1999,67(5):2125-2130.
11
Jin LJ, Ren L, Leung WK, et al. The in vivo expression of membrane-bound CD14 in periodontal health and disease. J Periodontol, 2004,75(4):578-585.
12
Gorska R, Gregorek H, Kowalski J, et al. Relationship between clinical parameters and cytokine profiles in inflamed gingival tissue and serum samples from patients with chronic periodontitis. J Clin Periodontol, 2003,30(12):1046-1052.
13
Dinarello CA. Biology of interleukin 1. FASEB, 1988,2(2):108-115.
14
Jandinski JJ, Stashenko P, Feder LS, et al. Localization of interleukin-1 beta in human periodontal tissue. J Periodontol,1991,62(1):36-43.
15
Dinarello CA. The biology of interleukin 1 and comparison to tumor necrosis factor. Immunol Lett, 1987,16(3-4):227-231.
16
Gemmell E, Seymour GJ. Cytokine profiles of cells extracted from humans with periodontal diseases. J Dent Res, 1998,77(1):16-26.
17
Rousset F, Garcia E, Defrance T, et al. Interleukin 10 is a potent growth and differentiation factor for activated human B lymphocytes. Proc Natl Acad Sci USA, 1992,89(5):1890-1893.
18
Yamazaki K, Tabeta K, Nakajima T, et al. Interleukin-10 gene promoter polymorphism in Japanese patients with adult and early-onset periodontitis. J Clin Periodontol, 2001,28(9):828-832.
19
Hirose M, Ishihara K, Saito A, et al. Expression of cytokines and inducible nitric oxide synthase in inflamed gingival tissue.J Periodontol, 2001,72(5):590-597.
20
Gamonal J, Acevedo A, Bascones A, et al. Levels of interleukin-1β,-8 and -10 and RANTES in gingival crevicular fluid and cell populations in adult periodontitis patients and the effect of periodontal treatment. J Periodontol, 2000,71(10):1535-1545.
21
Berkman N, John M, Roesems G, et al. Inhibition of macrophage inflammatory protein-1 alpha expression by IL-10.Differential sensitivities in human blood monocytes and alveolar macrophages. J Immunol, 1995,155(9):4412-4418.
22
Dickensheets HL, Donnelly RP. IFN-gamma and IL-10 inhibit induction of IL-1 receptor type I and type II gene expression by IL-4 and IL-13 in human monocytes. J Immunol, 1997,159(12):6226-6233.
23
Wang P, Wu P, Anthes JC, et al. Interleukin-10 inhibits interleukin-8 production in human neutrophils. Blood, 1994,83(9):2678-2683.
24
Deschner J, Arnold B, Kage A, et al. Suppression of interleukin-10 release from human periodontal ligament cells by interleukin-1beta in vitro. Arch Oral Biol, 2000,45(2):179-183.
[1] 沈梦晴, 吴婷, 王菲. 布地奈德联合特异性免疫治疗对支气管哮喘患儿1,25-二羟维生素D3及IL-6、IL-10水平的影响[J/OL]. 中华肺部疾病杂志(电子版), 2021, 14(01): 43-47.
[2] 张宇锋, 陈淑如, 张英. 急性肝衰竭大鼠血清TNF-α、IL-10水平的动态变化和TNF-α单克隆抗体护肝机制的探讨[J/OL]. 中华肝脏外科手术学电子杂志, 2012, 01(03): 191-195.
[3] 马春花, 罗京艺, 朱东华, 荆淑娟, 尚毅. 肠易安汤对溃疡性结肠炎大鼠溃疡组织中NF-κB p65及IL-10表达的影响[J/OL]. 中华结直肠疾病电子杂志, 2017, 06(02): 117-120.
[4] 陈客宏, 张炜炜, 张锦华, 杨聚荣, 李开龙, 张建国, 何娅妮. P2X4介导高糖刺激肾小管上皮细胞分泌白介素-1β的作用[J/OL]. 中华肾病研究电子杂志, 2012, 01(02): 109-115.
[5] 孙建军, 周杰, 李长栋, 荔志云. 异甘草素对颅脑损伤大鼠IL-6和IL-10表达的影响[J/OL]. 中华神经创伤外科电子杂志, 2021, 07(01): 12-16.
[6] 田锐, 谢云, 杜江, 金卫, 陆健, 谢晖, 朱献, 程瑞杰, 吕慧, 袁成斌, 王瑞兰. 热射病早期肠屏障功能损害与炎症反应因子的相关性[J/OL]. 中华重症医学电子杂志, 2018, 04(04): 333-337.
[7] 阳泽宇, 杨霞, 王嘉伟, 谭兴领, 张敏敏, 宁宗. GSDMD依赖性细胞焦亡在毒蛇咬伤患者中的表达[J/OL]. 中华临床医师杂志(电子版), 2021, 15(03): 187-190.
[8] 崔晓, 孙慧勤. 丁苯酞注射液对急性脑梗死患者血清IL-6、IL-1β、TNF-α及预后的影响[J/OL]. 中华脑血管病杂志(电子版), 2020, 14(01): 55-58.
阅读次数
全文


摘要