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中华口腔医学研究杂志(电子版) ›› 2020, Vol. 14 ›› Issue (05) : 288 -293. doi: 10.3877/cma.j.issn.1674-1366.2020.05.003

所属专题： 文献；

基础研究

原发性干燥综合征患者唇腺组织中白细胞介素7的表达及临床意义
林玉娇1, 王育凯2, 王友山1, 谢奕文1,†()   
  1. 1. 中山大学附属汕头医院·汕头市中心医院口腔科，汕头　515031
    2. 中山大学附属汕头医院·汕头市中心医院风湿免疫科，汕头　515031
  • 收稿日期:2020-05-12 出版日期:2020-10-01
  • 通信作者: 谢奕文

Expression and clinical significance of interleukin-7 in the salivary glands of patients with primary Sjögren′s syndrome

Yujiao Lin1, Yukai Wang2, Youshan Wang1, Yiwen Xie1,†()   

  1. 1. Department of Stomatology, Shantou Central Hospital, Shantou 515031, China
    2. Department of Rheumatology and Immunology, Shantou Central Hospital, Shantou 515031, China
  • Received:2020-05-12 Published:2020-10-01
  • Corresponding author: Yiwen Xie
  • About author:
    Corresponding author: Xie Yiwen, Email:
  • Supported by:
    Medical Scientific Research Foundation of Guangdong Province(A2019244); Medical Health Science and Technology Projects of Shantou(2015-123-07 and 2019-106-21)
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林玉娇, 王育凯, 王友山, 谢奕文. 原发性干燥综合征患者唇腺组织中白细胞介素7的表达及临床意义[J]. 中华口腔医学研究杂志(电子版), 2020, 14(05): 288-293.

Yujiao Lin, Yukai Wang, Youshan Wang, Yiwen Xie. Expression and clinical significance of interleukin-7 in the salivary glands of patients with primary Sjögren′s syndrome[J]. Chinese Journal of Stomatological Research(Electronic Edition), 2020, 14(05): 288-293.

目的

探讨原发性干燥综合征（pSS）患者唇腺组织中IL-7的表达及其与临床血清学参数间的关联。

方法

收集2015年1月至2017年12月汕头市中心医院pSS患者135例，以及同期唇外伤正常对照个体12例。免疫组织化学法检测两组个体唇腺组织中IL-7的表达，分别评估腺上皮细胞与浸润淋巴细胞IL-7的表达情况。Pearson相关分析唇腺组织IL-7的表达与抗核抗体（ANA）、C3、C4、C反应蛋白（CRP）、IgG等血清学指标间的关联性。

结果

IL-7在唇腺组织内淋巴细胞、腺泡导管及上皮细胞均可表达。浸润的淋巴细胞IL-7表达阳性率在pSS患者（88.14%，119/135）显著高于正常对照个体（8.3%，1/12），差异有统计学意义（χ2 = 46.82，P<0.001）。pSS患者唇腺组织中腺泡与导管上皮细胞与浸润淋巴细胞IL-7的表达相关（r = 0.27，P = 0.0012），未发现有浸润淋巴细胞IL-7表达与血清指标间的相关性。

结论

pSS患者唇腺组织中腺体上皮细胞、淋巴细胞IL-7异常高表达，且两者表达情况紧密相关，提示IL-7的表达可能参与了pSS腺体与淋巴细胞的相互作用，可作为pSS唇腺干预一个新的治疗靶点。

关键词: 干燥综合征, 白细胞介素7, 唇腺
Objective

To investigate the expression of interleukin-7 (IL-7) in the labial glands and its correlation with the clinical serum biomarkers in the patients with primary Sj?gren′s syndrome.

Methods

A total of 135 patients with pSS from the Department of Rheumatology and Immunology of Shantou Central Hospital from January 2015 to December 2017 were recruited, while 12 normal individuals in the same period were included as control. Immunohistochemical method was used to detect the expression level of IL-7 in the glandular epithelial cells and infiltrated lymphocytes. The relationships between IL-7 and serum ANA, C3, C4, CRP and IgG were investigated.

Results

IL-7 was highly expressed in the epithelial cells and lymphocytes infiltrated in the pSS salivary glands. The positive expression of IL-7 in the pSS patients (88.14%, 119/135) was significantly higher than that in the control (8.3%, 1/12; χ2 = 46.82, P<0.001) . The expression of IL-7 in the infiltrated lymphocytes was positively correlated with that in the epithelial cells (r = 0.27, P = 0.0012) , while no correlation with the serum biomarkers, including ANA, C3, C4, CRP and IgG was found.

Conclusions

The increased expression of IL-7 in the glandular epithelial cells in patients with pSS was related to its expression in infiltrated lymphocytes, indicating a crosstalk between epithelial cells and lymphocytes in the immunopathology of pSS. IL-7 can be a therapeutic target for pSS.

Key words: Sj?gren′s syndrome, Interleukin-7, Salivary glands, Labial
图2 白细胞介素7（IL-7）在原发性干燥综合征（pSS）患者唇腺组织腺体上皮细胞与浸润的淋巴细胞中的表达情况（高倍放大）2A：IL-7在腺体腺泡与导管上皮细胞中的低表达；2B：IL-7在腺体腺泡与导管上皮细胞中的高表达；2C：IL-7在浸润淋巴细胞中的不表达；2D：IL-7在浸润淋巴细胞中的高表达
图3 白细胞介素7（IL-7）在原发性干燥综合征（pSS）患者与正常唇腺组织浸润淋巴细胞中的H-score值的比较　A：浸润淋巴细胞IL-7表达H-score值在两组间的均值与标准差的比较；B：MedCalc计算的IL-7 H-score截点及其相对应的敏感性、特异性
图4 原发性干燥综合征（pSS）患者唇腺组织浸润的淋巴细胞白细胞介素7（IL-7）表达与唇腺上皮细胞及患者血清学间的相关性分析　A：浸润淋巴细胞与腺体上皮细胞IL-7表达H-score间存在线性相关，r = 0.27；B：浸润淋巴细胞IL-7表达H-score与抗核抗体（ANA）间不存在线性相关；C：浸润淋巴细胞IL-7表达H-score与补体C3间不存在线性相关；D：浸润淋巴细胞IL-7表达H-score与补体C4间不存在线性相关；E：浸润淋巴细胞IL-7表达H-score与超敏C反应蛋白（CRP）间不存在线性相关；F：浸润淋巴细胞IL-7表达H-score与IgG间不存在线性相关
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