目的

研究RNA 干扰技术（RNAi）下调牙周韧带（PDL）细胞中钙结合蛋白S100A₄ 的表达，获得稳定的细胞单克隆，拟为后续试验提供细胞水平平台。

方法

利用RNAi 技术，针对人cDNA序列设计了S100A₄ 干扰序列及一个阴性对照，将其连接到RNA 干扰载体pGenesil-1 上，转染人PDL 细胞，通过反转录聚合酶链反应（RT-PCR）和Western blot 检测有干扰序列的有效性。

结果

干扰片段转染72 h 后的mRNA 抑制效率为67.83%。

结论

RNAi 可以有效抑制PDL 细胞中S100A₄的基因表达，为进一步研究S100A₄ 在PDL 细胞中的功能提供新的思路。

Objective

Observation of using RNA interference technology by periodontal ligament（PDL） cells calcium binding protein S100A₄ expression， to obtain stable cell monoclonal， for follow-up tests provide cellular level platform.

Methods

Used the technology of RNA interference （RNAi） to cut down S100A₄ expression， targeting the coding sequence of human S100A₄ gene and a negative control were designed. Then they were ligated into the vector of pGenesil-1 respectively，and were transfected into PDL cells. By the means of RT-PCR and Western blot，we could examine their suppression.

Result

The results showed that the interference effects of the RNAi expression vectors were 67.83%.

Conclusion

RNA interference can be effectively suppressed in periodontal ligament cells and S100A₄ gene expression. This study provides a new means for investigating the effects of S100A₄ in PDL cells.