目的

探讨自噬诱导对舌鳞癌细胞增殖和迁移能力的影响。

方法

应用雷帕霉素（Rap）诱导上调舌鳞癌Tca8113 细胞自噬活性，Western blot 检测诱导后Tca8113 细胞自噬标记蛋白Beclin1 和LC3 的表达变化；MTT 法检测自噬诱导对细胞增殖的影响；Woundhealing 检测诱导后细胞迁移能力改变；SPSS 19.0 统计软件进行数据分析。

结果

Rap 诱导6 h 即可上调舌鳞癌Tca8113 细胞自噬标记蛋白LC3-Ⅱ表达，24 h 时LC3-Ⅱ表达最强，自噬活性最高（P ＜0.05）。 与对照组相比，Rap 诱导后细胞生长明显抑制（P ＜0.05），迁移速率减慢。

结论

上调舌鳞癌细胞自噬活性可以抑制其增殖和迁移。

Objective

To investigate the effect of autophagy induced by rapamycin on proliferation， migration of tongue squamous cell carcinoma （TSCC） cell line.

Methods

TSCC cell line （Tca8113） was treated with rapamycin to induce autophagy.Then， Western blot was performed to examine the expression of Beclin1 and LC3.MTT assay was used to evaluate cell proliferation.Wound healing was carried out to assess cell migration.

Results

After 6 h of Rapamycin treatment， the expression of LC3 was gradually increased in Tca8113 cell.After treatment with rapamycin for 24 h， LC3-Ⅱ was dramatically increased at protein level and autophagical activity was significantly increased （P ＜ 0.05）.MTT assay indicated that cell proliferation was inhibited by Rapamycin.Migration ability was also reduced after treatment with rapamycin （P ＜0.05）.

Conclusion

Rapamycin can induce autophagy and suppress proliferation and migration in tongue squamous cell line.