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中华口腔医学研究杂志(电子版) ›› 2011, Vol. 5 ›› Issue (02) : 156 -160. doi: 10.3877/cma.j.issn.1674-1366.2011.02.008

临床研究

EB 病毒与逆行性牙髓炎的相关性研究
高碧云1, 钟钰1, 陈文霞1,()   
  1. 1.530021 南宁,广西医科大学附属口腔医院牙体牙髓病科
  • 收稿日期:2011-02-28 出版日期:2011-04-01
  • 通信作者: 陈文霞
  • 基金资助:
    广西自然科学基金重点项目(2011GXNSFD018036)

Investigation on the association between Epstein-Barr virus and retrograde pulpitis

Bi-yun GAO1, Yu ZHONG1, Wen-xia CHEN1,()   

  1. 1.Department of Operative and Endodontics, College and Hospital of Stomatology, Guangxi Medical University, Nangning 530021, China
  • Received:2011-02-28 Published:2011-04-01
  • Corresponding author: Wen-xia CHEN
引用本文:

高碧云, 钟钰, 陈文霞. EB 病毒与逆行性牙髓炎的相关性研究[J/OL]. 中华口腔医学研究杂志(电子版), 2011, 5(02): 156-160.

Bi-yun GAO, Yu ZHONG, Wen-xia CHEN. Investigation on the association between Epstein-Barr virus and retrograde pulpitis[J/OL]. Chinese Journal of Stomatological Research(Electronic Edition), 2011, 5(02): 156-160.

目的

研究EB 病毒(EBV)与逆行性牙髓炎的相关性。

方法

应用巢式聚合酶链反应(nPCR)技术,检测30 例逆行性牙髓炎患者和30 例健康对照者牙髓组织中的EBV,比较分析它们的检出率。

结果

30 例逆行性牙髓炎患者的EBV 检出率为36.67%,显著高于健康对照者(0%),差异有统计学意义(P <0.01)。

结论

提示EBV 与逆行性牙髓炎有相关性。

Objective

To investigate the relationship between Epstein-Barr virus(EBV)and retrograde pulpitis.

Methods

Pulp tissue samples were collected from 30 patients of retrograde pulpitis and 30 healthy subjects.The nested polymerase chain reaction (nPCR) was used to examine EBV in pulp tissue.

Results

The positive rate of EBV detected in samples which were collected from the patients with retrograde pulpitis was significantly higher percentages(36.67%) compared with that from healthy pulp controls (0%, P <0.01).

Conclusions

EBV may be related to retrograde pulpitis.

图1 EBV 的特异性实验结果 1:DNA Marker; 2:EBV DNA; 3:HCMV MIE DNA; 4:HSV-1 DNA
图2 逆行性牙髓炎患者牙髓中EBV 巢式PCR 检测结果 1:DNA Marker; 2:EBV 阳性对照; 3:阴性对照; 4 ~6:逆行性牙髓炎患者牙髓中检测EBV 阳性者; 7 ~9:逆行性牙髓炎患者牙髓中检测EBV阴性者
表1 EBV 检测结果分析
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