目的

探讨电离辐射对血管内皮细胞分泌血管内皮生长因子（VEGF）、碱性成纤维生长因子（bFGF）和转化生长因子β1（TGF-β1）的影响。

方法

对人脐静脉血管内皮细胞（HUVEC）-12 以0、4、8、12 Gy 剂量⁶⁰Co γ 射线照射后培养12、24 h，采用流式细胞仪检测细胞VEGF、bFGF 和TGF-β1 的表达。

结果

VEGF 在4 Gy 照射后12 h 有较明显的增加，24 h后逐渐趋于正常；bFGF 在4 Gy 照射后12 和24 h 都有明显的表达，但表达量随着辐射剂量的增加而减少；TGF-β1 表达的增加呈剂量-时间依赖性。

结论

血管内皮细胞受电离辐射后VEGF、bFGF 和TGF-β1 的分泌均有增加，但三者的剂量-时间关系不一致，可能与细胞对辐射的反应和损伤抵抗作用不同有关。

Objective

To observe the effect of ionizing on the secretion of vasculare endothelial growth factor（VEGF）， basic fibroblast growth factor（bFGF） and transforming growth factor-β1 （TGF-β1） in vascular endothelial cell.

Methods

The expression levels of VEGF，bFGF and TGF-β1 of human umbilical vein endothelial cell （HUVEC）-12 were detected by flow cytometry at 12- and 24-hours post-irration with 0， 4， 8 and 12 Gy ⁶⁰Co γ-rays.

Results

VEGF increased significantly at 12 hours post-irration with 4 Gy and gradually tended to be normal level at 24 hours. The expression of bFGF increased significantly at 12- and 24-hour with 4 Gy but decreased depending on the increasing of radiation dose. The expression of TGF-β1 increased with the radiation doses and times.

Conclusion

The secretion of VEGF， bFGF and TGF-β1 of vascular endothelial cells increased but the relationships with the radiation dosage and time were not consistent. That may be related to the cellular reaction and damage resistance to the ionizing radiation.