目的

评估自体骨髓间充质干细胞（MSCs）种植珊瑚人工骨（NC）后形成的复合物结合牙周引导组织再生术治疗狗牙周缺损的效果。

方法

（1）体外分离获得狗的MSCs，矿化培养液进行成骨诱导，检测细胞的成骨活性；（2）将NC 和PLGA-NC 复合膜分别吸附MSCs 共培养，观察复合物的形态；（3）将MSCs 与NC 共培养10 d 后的复合物移植到狗的牙周实验性骨缺损处。 8 周后行组织学染色牙周再生检测。

结果

（1）诱导后的MSCs 成骨活性明显， 矿化结节茜素红染色和Ⅰ型胶原免疫组化染色阳性；（2）MSCs 与NC和PLGA-NC 复合膜均有较好的生物相容性；（3）MSCs-NC 复合物移植后可修复牙周缺损。

结论

MSCs 有望成为牙周前体细胞的体外来源，骨组织工程学方法修复牙周缺损是可行的。

Objective

To evaluate the effects of transplanting mesenchymal stem cells（MSCs） and natural coral （NC） complex combined with guided tissue regeneration （GTR） on the treatment of periodontal bone defects in the Mongrel dogs.

Methods

（1）MSCs isolated from the Mongrel dogs were cultured with mineralization medium for osteogenic differentiation. The osteogenic ability of cells was examined. （2）MSCs were seeded into porous NC particles and PLGA-NC complex membrane， the morphological characteristics of MSCs were observed with phase contrast microscope and scanning electron microscope. （3）After 10 days of co-culture of MSCs and NC， the complexes were transplanted into experimentally induced alveolar bone defects of dogs by GTR therapy using PLGA-NC membranes. The defects were histologically assessed 8 weeks later.

Results

（1）MSCs after induction showed osteogenic potential. The staining of mineralized nodules and type Ⅰcollagen were both positive. （2）Good biocompatibility was observed for MSCs and NC， as well as MSCs and the PLGA-NC membrane. （3）The defects were restored by transplanting with MSCs-NC complex.

Conclusions

MSCs was the potential precursor cells for periodontal tissue regeneration. It is indicated that bone tissue-engineering could be applied to restore periodontal defects.