目的

观察牙骨质附着蛋白（CAP）对体外培养的猴骨髓基质细胞增殖及矿化能力的影响。

方法

抽取猴髂骨骨髓， 全血培养法获得骨髓基质细胞。 培养液中CAP 的浓度分别为0.125、0.25、0.5、1、2 μg/ml，以不加CAP 为空白对照。 MTT 法测定各组细胞的增殖活性，同时检测细胞内碱性磷酸酶活性。 采用SAS 6.12 软件对实验数据行单因素方差分析。

结果

从第3 天开始，0.5、1、2 μg/ml CAP 组与对照组相比能显著促进细胞增殖。 对照组与不同浓度CAP 实验组对细胞内碱性磷酸酶合成差异无统计学意义。

结论

0.5 ～2 μg/ml 的CAP 都能显著促进体外培养的猴BMSCs 增殖，但各浓度组CAP 对细胞内碱性磷酸酶合成无影响。

Objective

The aim of this study is to investigate the effects of cementum attachment protein （CAP） on the proliferation and mineralization of cultured monkey bone marrow stromal cells （BMSCs）in vitro.

Methods

BMSCs were obtained from monkey bone marrow aspiration and cultured in DMEM medium with 10% FBS. The third passage cells were exposed to various concentrations of CAP （0.125， 0.25，0.5， 1 and 2 μg/ml）. Controls were BMSCs cultured in DMEM medium without CAP. BMSCs proliferation rates and alkaline phosphatase （ALP） synthesis were assessed by an MTT assay. Statistical analyses were performed with a parametric one- way analysis of variance （ANOVA） and Stument-Newman-Keuls test for multiple comparisons.

Results

The proliferation rate of BMSCs was significantly up-regulated by CAP at concentrations of 0.5 to 2 μg/ml （P＜0.05） compared with the control group. There was no significant difference between the control group and experimental groups in ALP synthesis.

Conclusion

CAP could promote the proliferating ability of monkey BMSCs in vitro， whereas have little effects on ALP synthesis.